Hotspot

accD

LSC · 63305-66475 · 3171 bp

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Pi 0.00158

MarkerSeek score 56.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 3171
variable_sites: 5
pi: 0.00158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGATGAAGACGCTTTTCGGA	GGCTGCTAGCTTCTTGACAA	45.0	50.0	57.3	58.2	326 bp	1.0	72.8
accD_p2	CAGATGAAGACGCTTTTCGG	GGCTGCTAGCTTCTTGACAA	50.0	50.0	57.2	58.2	327 bp	1.0	72.4
accD_p3	AGATGAAGACGCTTTTCGGAA	GGCTGCTAGCTTCTTGACAA	42.9	50.0	58.0	58.2	326 bp	1.0	70.4
accD_p4	AGATGAAGACGCTTTTCGGA	ACAAAATTTGCGGCAATCCA	45.0	40.0	57.3	57.5	310 bp	1.0	70.0
accD_p5	AGATGAAGACGCTTTTCGGA	GCAATCCATCTCGTGTTTGG	45.0	50.0	57.3	57.5	298 bp	1.0	69.9