Hotspot

rpl32-ndhF

IRa · 135884-137142 · 1259 bp

Back to Consolea

Pi 0.000794

MarkerSeek score 59.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: IRa
length_bp: 1259
variable_sites: 1
pi: 0.000794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTAACGCTGCCCAATATCCC	GAAGCGGAGTGAAGGGTATG	50.0	55.0	58.0	58.1	1396 bp	1.0	79.4
rpl32-ndhF_p2	TTAACGCTGCCCAATATCCC	GCGGAGTGAAGGGTATGATC	50.0	55.0	58.0	57.8	1393 bp	1.0	78.6
rpl32-ndhF_p3	AAAAGCTTTTAACGCTGCCC	GAAGCGGAGTGAAGGGTATG	45.0	55.0	57.9	58.1	1404 bp	1.0	78.4
rpl32-ndhF_p4	TTAACGCTGCCCAATATCCC	AGCGGAGTGAAGGGTATGAT	50.0	50.0	58.0	58.2	1394 bp	1.0	78.2
rpl32-ndhF_p5	AAAAGCTTTTAACGCTGCCC	GCGGAGTGAAGGGTATGATC	45.0	55.0	57.9	57.8	1401 bp	1.0	77.6