Hotspot

ndhG-trnL-UAG

IRb · 121581-121832 · 252 bp

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Pi 0.00397

MarkerSeek score 47.10

Primer available yes

Label ndhG-trnL-UAG

Feature Metadata

label_name: ndhG-trnL-UAG
feature_type: igs
region: IRb
length_bp: 252
variable_sites: 1
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-trnL-UAG_p1	TAGGTGCAATTTCTGTGGCT	CTCGCACTGCTTCCTAAGAG	45.0	55.0	57.4	58.1	331-332 bp	1.0	74.9
ndhG-trnL-UAG_p2	TAGGTGCAATTTCTGTGGCT	CTCTCGCACTGCTTCCTAAG	45.0	55.0	57.4	58.1	333-334 bp	1.0	74.9
ndhG-trnL-UAG_p3	TAGGTGCAATTTCTGTGGCT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.4	57.9	320-321 bp	1.0	74.6
ndhG-trnL-UAG_p4	TAGGTGCAATTTCTGTGGCT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.4	57.8	313-314 bp	1.0	74.1
ndhG-trnL-UAG_p5	TAGGTGCAATTTCTGTGGCT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.4	57.8	318-319 bp	1.0	74.0