Hotspot

trnI-CAU-ycf2

IRb · 88417-88551 · 135 bp

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Pi 0.00741
MarkerSeek score 56.80
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
135
variable_sites
1
pi
0.00741
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC GGGTCCATGAATCAGAGTCAG 50.0 52.4 58.0 57.8 282 bp 1.0 70.7
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC GGGTCCATGAATCAGAGTCA 50.0 50.0 58.0 56.6 282 bp 1.0 69.2
trnI-CAU-ycf2_p3 GTTGGGCGCTTTAACCATTC TGGGTCCATGAATCAGAGTC 50.0 50.0 58.0 56.6 283 bp 1.0 69.2
trnI-CAU-ycf2_p4 GTTGGGCGCTTTAACCATTC TGGGTCCATGAATCAGAGTCA 50.0 47.6 58.0 58.4 283 bp 1.0 68.6
trnI-CAU-ycf2_p5 GTTGGGCGCTTTAACCATTC AGGTTCTTTTGATTGGTCCGA 50.0 42.9 58.0 57.5 206 bp 1.0 68.3