Hotspot

ndhK-ndhC

LSC · 56308-56365 · 58 bp

Back to Consolea

Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	CGATCAAGTAGGGGAAACTCA	CATGGCGAAAAGGAGCATTG	47.6	50.0	57.1	58.1	122 bp	1.0	64.9
ndhK-ndhC_p2	CGATCAAGTAGGGGAAACTCA	TGGGCAATGAGTTTCGATGT	47.6	45.0	57.1	57.8	210 bp	1.0	63.7
ndhK-ndhC_p3	TCGATCAAGTAGGGGAAACTC	CATGGCGAAAAGGAGCATTG	47.6	50.0	56.8	58.1	123 bp	1.0	62.7
ndhK-ndhC_p4	CGATCAAGTAGGGGAAACTCAA	CATGGCGAAAAGGAGCATTG	45.5	50.0	57.8	58.1	122 bp	1.0	62.4
ndhK-ndhC_p5	TCGATCAAGTAGGGGAAACTC	TGGGCAATGAGTTTCGATGT	47.6	45.0	56.8	57.8	211 bp	1.0	61.5