Hotspot

rpoB-trnC-GCA

LSC · 30902-32042 · 1141 bp

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Pi 0

MarkerSeek score 59.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTTCATCCCGTAGCA	ATTAGAATCAGGCGACACCC	47.6	50.0	58.1	57.4	1237-1243 bp	1.0	66.8
rpoB-trnC-GCA_p2	CCTCATTTTCATCCCGTAGCA	CGACACCCAGATTCGAACTG	47.6	55.0	58.1	58.6	1225-1231 bp	1.0	66.5
rpoB-trnC-GCA_p3	CCTCATTTTCATCCCGTAGCA	TTAGAATCAGGCGACACCCA	47.6	50.0	58.1	58.7	1236-1242 bp	1.0	65.9
rpoB-trnC-GCA_p4	TTCCCTCATTTTCATCCCGT	ATTAGAATCAGGCGACACCC	45.0	50.0	56.8	57.4	1240-1246 bp	1.0	65.9
rpoB-trnC-GCA_p5	TTCCCTCATTTTCATCCCGT	CGACACCCAGATTCGAACTG	45.0	55.0	56.8	58.6	1228-1234 bp	1.0	65.6