primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	rps8	1	AAATTCGGAGACACCACTGA	GGGACCAACTAATAGGGGGA	20	20	45.0	55.0	56.8	57.8	0.0	6.5	1.423112	3809	4213	526-526	1.000000	68.9
rps8_p2	rps8	2	TCGGAGACACCACTGATTTT	GGGACCAACTAATAGGGGGA	20	20	45.0	55.0	56.8	57.8	0.0	6.5	1.423112	3809	4213	522-522	1.000000	68.9
rps8_p3	rps8	3	TTCGGAGACACCACTGATTT	GGGACCAACTAATAGGGGGA	20	20	45.0	55.0	56.8	57.8	0.0	6.5	1.423112	3809	4213	523-523	1.000000	68.9
rps8_p4	rps8	4	TCGGAGACACCACTGATTTT	AGGGACCAACTAATAGGGGG	20	20	45.0	55.0	56.8	57.8	0.0	0.0	1.429516	3809	4213	523-523	1.000000	68.9
rps8_p5	rps8	5	TTCGGAGACACCACTGATTT	AGGGACCAACTAATAGGGGG	20	20	45.0	55.0	56.8	57.8	0.0	0.0	1.429516	3809	4213	524-524	1.000000	68.9
trnG-UCC_p1	trnG-UCC	1	TCGACAAAAGGTTCAGCTCA	TGGATTGTACGCGAGATGAG	20	20	45.0	50.0	57.4	57.5	0.0	15.9	1.105045	13303	14066	880-881	1.000000	71.3
trnG-UCC_p2	trnG-UCC	2	AGCTCGACAAAAGGTTCAGC	TGGATTGTACGCGAGATGAG	20	20	50.0	50.0	58.8	57.5	0.0	15.9	1.261696	13303	14066	883-884	1.000000	70.0
trnG-UCC_p3	trnG-UCC	3	GCTCGACAAAAGGTTCAGCT	TGGATTGTACGCGAGATGAG	20	20	50.0	50.0	58.8	57.5	0.0	15.9	1.261696	13303	14066	882-883	1.000000	70.0
trnG-UCC_p4	trnG-UCC	4	TCGACAAAAGGTTCAGCTCA	GGATTGTACGCGAGATGAGA	20	20	45.0	50.0	57.4	57.2	0.0	15.9	1.389338	13303	14066	879-880	1.000000	69.0
trnG-UCC_p5	trnG-UCC	5	GCTCGACAAAAGGTTCAGCT	GGATTGTACGCGAGATGAGA	20	20	50.0	50.0	58.8	57.2	0.0	15.9	1.545988	13303	14066	881-882	1.000000	67.7
atpF_p1	atpF	1	AGGGTTGCCATGAGTCTTTC	GTAGCAAAGAGGTGAGGCAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391580	15971	17278	1441-1445	1.000000	77.1
atpF_p2	atpF	2	GAAGGGTTGCCATGAGTCTT	GTAGCAAAGAGGTGAGGCAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391580	15971	17278	1443-1447	1.000000	77.1
atpF_p3	atpF	3	GGGTTGCCATGAGTCTTTCT	GTAGCAAAGAGGTGAGGCAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391580	15971	17278	1440-1444	1.000000	77.1
atpF_p4	atpF	4	AGGGTTGCCATGAGTCTTTC	GAGGTGAGGCAATACAACGA	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.586660	15971	17278	1433-1437	1.000000	75.5
atpF_p5	atpF	5	GGGTTGCCATGAGTCTTTCT	GAGGTGAGGCAATACAACGA	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.586660	15971	17278	1432-1436	1.000000	75.5
rpoC2_p1	rpoC2	1	CGCCTCTTTGCCAATAAATCA	ACTGATGTGTACCATGCACC	21	20	42.9	50.0	57.2	57.9	0.0	0.0	1.894939	23074	23193	335-335	1.000000	65.1
rpoC2_p2	rpoC2	2	CGCCTCTTTGCCAATAAATCA	ATCAGGAAGGCCGTACAGAT	21	20	42.9	50.0	57.2	58.2	0.0	2.9	1.986364	23074	23193	258-258	1.000000	64.4
rpoC2_p3	rpoC2	3	CGCCTCTTTGCCAATAAATCA	ACCATGCACCCGAATTTACA	21	20	42.9	45.0	57.2	57.8	0.0	0.0	1.989211	23074	23193	325-325	1.000000	64.3
rpoC2_p4	rpoC2	4	CGCCTCTTTGCCAATAAATCA	TGTACCATGCACCCGAATTT	21	20	42.9	45.0	57.2	57.8	0.0	0.0	1.989211	23074	23193	328-328	1.000000	64.3
rpoC2_p5	rpoC2	5	CGCCTCTTTGCCAATAAATCA	GGGGAAATGCACTGGAGTAC	21	20	42.9	55.0	57.2	58.2	0.0	0.0	2.029641	23074	23193	353-353	1.000000	64.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCTCATTTTCATCCCGTAGCA	ATTAGAATCAGGCGACACCC	21	20	47.6	50.0	58.1	57.4	0.0	0.0	1.704131	30902	32042	1237-1243	1.000000	66.8
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCTCATTTTCATCCCGTAGCA	CGACACCCAGATTCGAACTG	21	20	47.6	55.0	58.1	58.6	0.0	16.2	1.731874	30902	32042	1225-1231	1.000000	66.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCTCATTTTCATCCCGTAGCA	TTAGAATCAGGCGACACCCA	21	20	47.6	50.0	58.1	58.7	0.0	0.0	1.810211	30902	32042	1236-1242	1.000000	65.9
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTTCATCCCGT	ATTAGAATCAGGCGACACCC	20	20	45.0	50.0	56.8	57.4	0.0	0.0	1.816017	30902	32042	1240-1246	1.000000	65.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCCCTCATTTTCATCCCGT	CGACACCCAGATTCGAACTG	20	20	45.0	55.0	56.8	58.6	0.0	16.2	1.843761	30902	32042	1228-1234	1.000000	65.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	20	20	55.0	55.0	58.1	58.5	7.6	0.0	0.582197	36525	37821	1431-1432	1.000000	75.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	TCCCTCCGTAACCAGTCATC	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.604351	36525	37821	1434-1435	1.000000	75.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	TCCCTCCGTAACCAGTCATC	20	20	50.0	55.0	58.3	58.5	0.0	0.0	0.800935	36525	37821	1408-1409	1.000000	73.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GGCCCTTTTAACTCAGTGGT	TCCCTCCGTAACCAGTCATC	20	20	50.0	55.0	57.7	58.5	0.0	0.0	0.806106	36525	37821	1446-1447	1.000000	73.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	GCAATAGACCAGACCAACCT	20	20	55.0	50.0	58.1	57.2	7.6	0.0	0.853476	36525	37821	1466-1467	1.000000	73.2
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TCCCGATGGTTGGTCAAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.522920	41156	41522	517-517	1.000000	76.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTTGCTTCTCCCGATGGTTG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.5	57.8	0.0	1.1	0.636207	41156	41522	525-525	1.000000	75.1
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CCCGATGGTTGGTCAAGTAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.5	57.8	0.0	1.1	0.652847	41156	41522	516-516	1.000000	74.9
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	ATTTGCTTCTCCCGATGGTT	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	57.5	57.8	0.0	1.1	0.684291	41156	41522	526-526	1.000000	74.7
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CGTTGTATTTGCTTCTCCCG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.2	57.8	0.0	1.1	0.976120	41156	41522	532-532	1.000000	72.3
ndhK_p1	ndhK	1	AATTGACCCGCTGCTTAACT	AAACAAATCAGATTGAAACAGT	20	22	45.0	27.3	57.8	52.8	0.0	0.0	7.438273	55630	56307	745-745	1.000000	40.0
ndhK_p2	ndhK	2	AATTGACCCGCTGCTTAACT	AACAAATCAGATTGAAACAGTT	20	22	45.0	27.3	57.8	52.8	0.0	0.0	7.438273	55630	56307	744-744	1.000000	40.0
ndhK_p3	ndhK	3	ATTGACCCGCTGCTTAACTT	AAACAAATCAGATTGAAACAGT	20	22	45.0	27.3	57.8	52.8	0.0	0.0	7.438273	55630	56307	744-744	1.000000	40.0
ndhK_p4	ndhK	4	ATTGACCCGCTGCTTAACTT	AACAAATCAGATTGAAACAGTT	20	22	45.0	27.3	57.8	52.8	0.0	0.0	7.438273	55630	56307	743-743	1.000000	40.0
ndhK_p5	ndhK	5	ATTGACCCGCTGCTTAACTT	AAACAAATCAGATTGAAACAGTT	20	23	45.0	26.1	57.8	53.6	0.0	0.0	7.589518	55630	56307	744-744	1.000000	40.0
ndhK-ndhC_p1	ndhK-ndhC	1	CGATCAAGTAGGGGAAACTCA	CATGGCGAAAAGGAGCATTG	21	20	47.6	50.0	57.1	58.1	0.0	0.0	1.970348	56308	56365	122-122	1.000000	64.9
ndhK-ndhC_p2	ndhK-ndhC	2	CGATCAAGTAGGGGAAACTCA	TGGGCAATGAGTTTCGATGT	21	20	47.6	45.0	57.1	57.8	0.0	0.0	2.086448	56308	56365	210-210	1.000000	63.7
ndhK-ndhC_p3	ndhK-ndhC	3	TCGATCAAGTAGGGGAAACTC	CATGGCGAAAAGGAGCATTG	21	20	47.6	50.0	56.8	58.1	0.0	0.0	2.248502	56308	56365	123-123	1.000000	62.7
ndhK-ndhC_p4	ndhK-ndhC	4	CGATCAAGTAGGGGAAACTCAA	CATGGCGAAAAGGAGCATTG	22	20	45.5	50.0	57.8	58.1	0.0	0.0	2.284973	56308	56365	122-122	1.000000	62.4
ndhK-ndhC_p5	ndhK-ndhC	5	TCGATCAAGTAGGGGAAACTC	TGGGCAATGAGTTTCGATGT	21	20	47.6	45.0	56.8	57.8	0.0	0.0	2.364602	56308	56365	211-211	1.000000	61.5
accD_p1	accD	1	AGATGAAGACGCTTTTCGGA	GGCTGCTAGCTTCTTGACAA	20	20	45.0	50.0	57.3	58.2	0.0	17.2	0.930122	64290	64409	326-326	1.000000	72.8
accD_p2	accD	2	CAGATGAAGACGCTTTTCGG	GGCTGCTAGCTTCTTGACAA	20	20	50.0	50.0	57.2	58.2	0.0	17.2	0.981608	64290	64409	327-327	1.000000	72.4
accD_p3	accD	3	AGATGAAGACGCTTTTCGGAA	GGCTGCTAGCTTCTTGACAA	21	20	42.9	50.0	58.0	58.2	0.0	17.2	1.226634	64290	64409	326-326	1.000000	70.4
accD_p4	accD	4	AGATGAAGACGCTTTTCGGA	ACAAAATTTGCGGCAATCCA	20	20	45.0	40.0	57.3	57.5	0.0	17.2	1.283900	64290	64409	310-310	1.000000	70.0
accD_p5	accD	5	AGATGAAGACGCTTTTCGGA	GCAATCCATCTCGTGTTTGG	20	20	45.0	50.0	57.3	57.5	0.0	3.8	1.293269	64290	64409	298-298	1.000000	69.9
psbE-rps12_p1	psbE-rps12	1	CAACCCGCAATGAATAGGGA	CGGGGGATTTCGAGACATTT	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.089721	72784	73951	1339-1339	1.000000	79.3
psbE-rps12_p2	psbE-rps12	2	AGAACGTTCTCCTGTGCTTC	CGGGGGATTTCGAGACATTT	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.194730	72784	73951	1262-1262	1.000000	78.5
psbE-rps12_p3	psbE-rps12	3	ACGAATAACCAACCCGCAAT	CGGGGGATTTCGAGACATTT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.209408	72784	73951	1348-1348	1.000000	78.4
psbE-rps12_p4	psbE-rps12	4	TGCTGACGAATAACCAACCC	CGGGGGATTTCGAGACATTT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.222312	72784	73951	1353-1353	1.000000	78.3
psbE-rps12_p5	psbE-rps12	5	CGAATAACCAACCCGCAATG	CGGGGGATTTCGAGACATTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.225738	72784	73951	1347-1347	1.000000	78.3
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	GGGTCCATGAATCAGAGTCAG	20	21	50.0	52.4	58.0	57.8	7.6	6.7	1.200279	88417	88551	282-282	1.000000	70.7
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GGGTCCATGAATCAGAGTCA	20	20	50.0	50.0	58.0	56.6	7.6	2.4	1.390195	88417	88551	282-282	1.000000	69.2
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	TGGGTCCATGAATCAGAGTC	20	20	50.0	50.0	58.0	56.6	7.6	0.0	1.390195	88417	88551	283-283	1.000000	69.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	TGGGTCCATGAATCAGAGTCA	20	21	50.0	47.6	58.0	58.4	7.6	2.4	1.459025	88417	88551	283-283	1.000000	68.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	AGGTTCTTTTGATTGGTCCGA	20	21	50.0	42.9	58.0	57.5	7.6	0.0	1.511324	88417	88551	206-206	1.000000	68.3
trnI_p1	trnI	1	AGAGAGGGATGGGGTTTCTC	TCTTATTCTTTTCCCCGGCG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.146258	101256	102277	1109-1109	1.000000	78.9
trnI_p2	trnI	2	AGAGGGATGGGGTTTCTCTC	TCTTATTCTTTTCCCCGGCG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.146258	101256	102277	1107-1107	1.000000	78.9
trnI_p3	trnI	3	GAGAGGGATGGGGTTTCTCT	TCTTATTCTTTTCCCCGGCG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.146258	101256	102277	1108-1108	1.000000	78.9
trnI_p4	trnI	4	GATGGGGTTTCTCTCGCTTT	TCTTATTCTTTTCCCCGGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148023	101256	102277	1102-1102	1.000000	78.9
trnI_p5	trnI	5	AGAGGGATGGGGTTTCTCTC	GCCAAGTGGAGCATGTATGA	20	20	55.0	50.0	58.1	58.0	0.0	0.2	0.156264	101256	102277	1149-1149	1.000000	78.8
ndhF-rpl32_p1	ndhF-rpl32	1	GAAGCGGAGTGAAGGGTATG	TTAACGCTGCCCAATATCCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.083015	110413	111671	1396-1396	1.000000	79.4
ndhF-rpl32_p2	ndhF-rpl32	2	GCGGAGTGAAGGGTATGATC	TTAACGCTGCCCAATATCCC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.180683	110413	111671	1393-1393	1.000000	78.6
ndhF-rpl32_p3	ndhF-rpl32	3	GAAGCGGAGTGAAGGGTATG	AAAAGCTTTTAACGCTGCCC	20	20	55.0	45.0	58.1	57.9	0.0	22.7	0.207591	110413	111671	1404-1404	1.000000	78.4
ndhF-rpl32_p4	ndhF-rpl32	4	AGCGGAGTGAAGGGTATGAT	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.229626	110413	111671	1394-1394	1.000000	78.2
ndhF-rpl32_p5	ndhF-rpl32	5	GCGGAGTGAAGGGTATGATC	AAAAGCTTTTAACGCTGCCC	20	20	55.0	45.0	57.8	57.9	0.0	22.7	0.305259	110413	111671	1401-1401	1.000000	77.6
ndhG-trnL-UAG_p1	ndhG-trnL-UAG	1	TAGGTGCAATTTCTGTGGCT	CTCGCACTGCTTCCTAAGAG	20	20	45.0	55.0	57.4	58.1	0.0	0.0	0.664917	121581	121832	331-332	1.000000	74.9
ndhG-trnL-UAG_p2	ndhG-trnL-UAG	2	TAGGTGCAATTTCTGTGGCT	CTCTCGCACTGCTTCCTAAG	20	20	45.0	55.0	57.4	58.1	0.0	0.0	0.664917	121581	121832	333-334	1.000000	74.9
ndhG-trnL-UAG_p3	ndhG-trnL-UAG	3	TAGGTGCAATTTCTGTGGCT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.709112	121581	121832	320-321	1.000000	74.6
ndhG-trnL-UAG_p4	ndhG-trnL-UAG	4	TAGGTGCAATTTCTGTGGCT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.4	57.8	0.0	0.0	0.769839	121581	121832	313-314	1.000000	74.1
ndhG-trnL-UAG_p5	ndhG-trnL-UAG	5	TAGGTGCAATTTCTGTGGCT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.4	57.8	0.0	0.0	0.781130	121581	121832	318-319	1.000000	74.0
rpl32-ndhF_p1	rpl32-ndhF	1	TTAACGCTGCCCAATATCCC	GAAGCGGAGTGAAGGGTATG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.083015	135884	137142	1396-1396	1.000000	79.4
rpl32-ndhF_p2	rpl32-ndhF	2	TTAACGCTGCCCAATATCCC	GCGGAGTGAAGGGTATGATC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.180683	135884	137142	1393-1393	1.000000	78.6
rpl32-ndhF_p3	rpl32-ndhF	3	AAAAGCTTTTAACGCTGCCC	GAAGCGGAGTGAAGGGTATG	20	20	45.0	55.0	57.9	58.1	22.7	0.0	0.207591	135884	137142	1404-1404	1.000000	78.4
rpl32-ndhF_p4	rpl32-ndhF	4	TTAACGCTGCCCAATATCCC	AGCGGAGTGAAGGGTATGAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.229626	135884	137142	1394-1394	1.000000	78.2
rpl32-ndhF_p5	rpl32-ndhF	5	AAAAGCTTTTAACGCTGCCC	GCGGAGTGAAGGGTATGATC	20	20	45.0	55.0	57.9	57.8	22.7	0.0	0.305259	135884	137142	1401-1401	1.000000	77.6
trnI_p1	trnI	1	TCTTATTCTTTTCCCCGGCG	AGAGAGGGATGGGGTTTCTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.146258	145278	146299	1109-1109	1.000000	78.9
trnI_p2	trnI	2	TCTTATTCTTTTCCCCGGCG	AGAGGGATGGGGTTTCTCTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.146258	145278	146299	1107-1107	1.000000	78.9
trnI_p3	trnI	3	TCTTATTCTTTTCCCCGGCG	GAGAGGGATGGGGTTTCTCT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.146258	145278	146299	1108-1108	1.000000	78.9
trnI_p4	trnI	4	TCTTATTCTTTTCCCCGGCG	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.148023	145278	146299	1102-1102	1.000000	78.9
trnI_p5	trnI	5	GCCAAGTGGAGCATGTATGA	AGAGGGATGGGGTTTCTCTC	20	20	50.0	55.0	58.0	58.1	0.2	0.0	0.156264	145278	146299	1149-1149	1.000000	78.8