Hotspot

accD

LSC · 56109-57566 · 1458 bp

Back to Conringia

Pi 0.0344

MarkerSeek score 65.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 50
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTTAAGTTCCGTGTCAGGGC	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.1	58.2	2632-2649 bp	1.0	80.8
accD_p2	CCATCCTGCATGTTGTCCTT	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.2	58.2	2584-2601 bp	1.0	80.5
accD_p3	GCATCGATTGCGTACTCTGA	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.2	58.2	2328-2346 bp	1.0	80.5
accD_p4	TTTAAGTTCCGTGTCAGGGC	CAAGTGCCGGAAAGACTAGG	50.0	55.0	58.1	58.3	2638-2655 bp	1.0	80.4
accD_p5	TTTAAGTTCCGTGTCAGGGC	CGGGAAATGCACAAAGATGG	50.0	50.0	58.1	57.7	2162-2167 bp	1.0	80.0