Hotspot

rps15-ycf1

SSC · 122237-124502 · 2266 bp

Back to Conringia

Pi 0.0438

MarkerSeek score 68.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 2266
variable_sites: 99
pi: 0.0438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	GGACAAATTCTTCACTCAGCG	50.0	47.6	58.0	57.5	2823-2825 bp	1.0	71.8
rps15-ycf1_p2	GATCCCCTGCTTTCGTCTTT	GGACAAATTCTTCACTCAGCG	50.0	47.6	57.9	57.5	2693-2695 bp	1.0	71.0
rps15-ycf1_p3	ATAAGCCAGCAGTCGTTGAC	GGACAAATTCTTCACTCAGCG	50.0	47.6	58.3	57.5	2829-2831 bp	1.0	69.6
rps15-ycf1_p4	TCGTTGACGTTTTCCCAGAA	GGACAAATTCTTCACTCAGCG	45.0	47.6	57.7	57.5	2817-2819 bp	1.0	69.3
rps15-ycf1_p5	ACAAATAAGCCAGCAGTCGT	GGACAAATTCTTCACTCAGCG	45.0	47.6	57.8	57.5	2833 bp	0.5	46.8