Hotspot

trnL-CUA-ccsA

SSC · 113033-113150 · 118 bp

Back to Conringia

Pi 0.0619

MarkerSeek score 75.00

Primer available yes

Label trnL-CUA-ccsA

Feature Metadata

label_name: trnL-CUA-ccsA
feature_type: igs
region: SSC
length_bp: 118
variable_sites: 7
pi: 0.0619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CUA-ccsA_p1	TTTGAACAGGTTGCCGCTAT	TGCAGACTGAGGCATTTTGT	45.0	45.0	58.1	58.0	589-594 bp	1.0	81.1
trnL-CUA-ccsA_p2	TAGACACGCTGCTCTTAGGA	TGCAGACTGAGGCATTTTGT	50.0	45.0	57.9	58.0	558-563 bp	1.0	80.9
trnL-CUA-ccsA_p3	TTTGAACAGGTTGCCGCTAT	TAACCACTGGGACTGGAGAG	45.0	55.0	58.1	58.1	622-627 bp	1.0	80.7
trnL-CUA-ccsA_p4	TAGACACGCTGCTCTTAGGA	TAACCACTGGGACTGGAGAG	50.0	55.0	57.9	58.1	591-596 bp	1.0	80.4
trnL-CUA-ccsA_p5	AAATTGGTAGACACGCTGCT	TGCAGACTGAGGCATTTTGT	45.0	45.0	57.8	58.0	565-570 bp	1.0	80.4