Hotspot

rpl32-trnL-CUA

SSC · 111993-112952 · 960 bp

Back to Conringia

Pi 0.084

MarkerSeek score 82.90

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 960
variable_sites: 68
pi: 0.084
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	TCGTTGAAAGCTTTTTCGTTAGG	TCCTAAGAGCAGCGTGTCTA	39.1	50.0	58.2	57.9	952-1077 bp	1.0	63.1
rpl32-trnL-CUA_p2	GTTGAAAGCTTTTTCGTTAGGGA	TCCTAAGAGCAGCGTGTCTA	39.1	50.0	57.8	57.9	950-1075 bp	1.0	63.0
rpl32-trnL-CUA_p3	CGTTGAAAGCTTTTTCGTTAGG	TCCTAAGAGCAGCGTGTCTA	40.9	50.0	56.8	57.9	951-1076 bp	1.0	62.9
rpl32-trnL-CUA_p4	TCGTTGAAAGCTTTTTCGTTAGG	AGCAGCGTGTCTACCAATTT	39.1	45.0	58.2	57.8	945-1070 bp	1.0	62.6
rpl32-trnL-CUA_p5	TCGTTGAAAGCTTTTTCGTTAGG	CTAAGAGCAGCGTGTCTACC	39.1	55.0	58.2	57.8	950-1075 bp	1.0	62.5