Hotspot

petA-psbJ

LSC · 61602-62213 · 612 bp

Back to Conringia

Pi 0.0447

MarkerSeek score 81.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 27
pi: 0.0447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.0	58.1	800-806 bp	1.0	74.4
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.0	57.8	801-807 bp	1.0	73.7
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	797-803 bp	1.0	73.3
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	798-804 bp	1.0	72.6
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	795-801 bp	1.0	71.9