Hotspot

trnF-UUC-ndhJ

LSC · 47356-47925 · 570 bp

Back to Conringia

Pi 0.0626

MarkerSeek score 83.10

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 34
pi: 0.0626
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	675-682 bp	1.0	86.5
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	674-681 bp	1.0	86.5
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	702-709 bp	1.0	86.4
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	50.0	50.0	58.1	57.9	699-706 bp	1.0	85.9
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	50.0	50.0	58.1	58.2	709-716 bp	1.0	84.6