Hotspot

trnS-UCC-rps4

LSC · 44250-44524 · 275 bp

Back to Conringia

Pi 0.0582

MarkerSeek score 77.50

Primer available yes

Label trnS-UCC-rps4

Feature Metadata

label_name: trnS-UCC-rps4
feature_type: igs
region: LSC
length_bp: 275
variable_sites: 16
pi: 0.0582
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UCC-rps4_p1	GAGTGGTTGAAGGCGTAACA	AGGAAATGCGTCGGTTTGAA	50.0	45.0	58.1	58.4	416-421 bp	1.0	78.8
trnS-UCC-rps4_p2	GAGTGGTTGAAGGCGTAACA	GGAAATGCGTCGGTTTGAAA	50.0	45.0	58.1	57.6	415-420 bp	1.0	78.8
trnS-UCC-rps4_p3	GAGTGGTTGAAGGCGTAACA	TAGGAAATGCGTCGGTTTGA	50.0	45.0	58.1	57.5	417-422 bp	1.0	78.4
trnS-UCC-rps4_p4	TCCTGATGTCGGAGAGATGG	AGGAAATGCGTCGGTTTGAA	55.0	45.0	58.3	58.4	438-443 bp	1.0	77.3
trnS-UCC-rps4_p5	TCCTGATGTCGGAGAGATGG	GGAAATGCGTCGGTTTGAAA	55.0	45.0	58.3	57.6	437-442 bp	1.0	77.2