Hotspot

atpF-atpH

LSC · 12614-13108 · 495 bp

Back to Conringia

Pi 0.0498

MarkerSeek score 74.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 24
pi: 0.0498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GAAAGAATCGGTTAAATTTTTCA	TGGCCTGGTTGTAGCATTAG	26.1	50.0	52.3	57.6	565-568 bp	1.0	45.2
atpF-atpH_p2	GAAAGAATCGGTTAAATTTTTCAT	TGGCCTGGTTGTAGCATTAG	25.0	50.0	52.9	57.6	565-568 bp	1.0	45.2
atpF-atpH_p3	GAAAGAATCGGTTAAATTTTTCA	TTTATGGCCTGGTTGTAGCA	26.1	45.0	52.3	57.1	569-572 bp	1.0	45.2
atpF-atpH_p4	GAAAGAATCGGTTAAATTTTTCA	GGCCTGGTTGTAGCATTAGC	26.1	55.0	52.3	59.0	564-567 bp	1.0	45.2
atpF-atpH_p5	GAAAGAATCGGTTAAATTTTTCA	ATGGCCTGGTTGTAGCATTAG	26.1	47.6	52.3	58.1	566-569 bp	1.0	45.2