Hotspot

trnK-AAA-rps16

LSC · 4381-5026 · 646 bp

Back to Conringia

Pi 0.0669

MarkerSeek score 77.20

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 42
pi: 0.0669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	981-1045 bp	1.0	84.4
trnK-AAA-rps16_p2	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	982-1046 bp	1.0	84.4
trnK-AAA-rps16_p3	CCTTTCAGGATCAGTCGTGG	ATGCGTGAATCAAGCGTTTG	55.0	45.0	58.0	58.0	1486-1571 bp	1.0	83.9
trnK-AAA-rps16_p4	CCTTTCAGGATCAGTCGTGG	CATGCGTGAATCAAGCGTTT	55.0	45.0	58.0	58.0	1487-1572 bp	1.0	83.9
trnK-AAA-rps16_p5	TCAGGATCAGTCGTGGTCTT	ATGCGTGAATCAAGCGTTTG	50.0	45.0	58.1	58.0	1482-1567 bp	1.0	83.4