Hotspot

psbA-trnK-AAA

LSC · 1466-1744 · 279 bp

Back to Conringia

Pi 0.043

MarkerSeek score 74.60

Primer available yes

Label psbA-trnK-AAA

Feature Metadata

label_name: psbA-trnK-AAA
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 11
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-AAA_p1	ACGGTTTTCAGTGCTGGTTA	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.7	58.4	449-470 bp	1.0	79.9
psbA-trnK-AAA_p2	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	379-402 bp	1.0	78.7
psbA-trnK-AAA_p3	ACGGTTTTCAGTGCTGGTTA	TGAAAAATGCAAGCACGGTT	45.0	40.0	57.7	57.4	452-473 bp	1.0	78.5
psbA-trnK-AAA_p4	TACAGAAGCGACCCCATAGG	AAAATGCAAGCACGGTTTGG	55.0	45.0	58.6	58.4	423-444 bp	1.0	78.0
psbA-trnK-AAA_p5	ATCCAGTTACAGAAGCGACC	AAAATGCAAGCACGGTTTGG	50.0	45.0	57.3	58.4	430-451 bp	1.0	77.2