Hotspot

rps14

Genome · 43070-43372 · 303 bp

Back to Conocephalum

Pi 0

MarkerSeek score 20.60

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	GGATTCGAACCTGTGACCTC	GCTCATGAGCGTACTCCTTT	55.0	50.0	58.0	57.7	713-722 bp	1.0	79.8
rps14_p2	AAGGTTATGAGCCTTGCGAG	GCTCATGAGCGTACTCCTTT	50.0	50.0	58.0	57.7	693-702 bp	1.0	79.8
rps14_p3	GGATTCGAACCTGTGACCTC	TCTGTTTGGGCATGGATGTT	55.0	45.0	58.0	57.7	824-833 bp	1.0	79.6
rps14_p4	GGATTCGAACCTGTGACCTC	TTCTGTTTGGGCATGGATGT	55.0	45.0	58.0	57.7	825-834 bp	1.0	79.6
rps14_p5	AAGGTTATGAGCCTTGCGAG	TCTGTTTGGGCATGGATGTT	50.0	45.0	58.0	57.7	804-813 bp	1.0	79.5