Hotspot

psaJ-rpl33

Genome · 65641-65754 · 114 bp

Back to Conocephalum

Pi 0.0614

MarkerSeek score 76.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 114
variable_sites: 7
pi: 0.0614
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACTGCACCTGTTTTAGCTACA	TTTTCATCATTTTGAGCACAA	42.9	28.6	57.5	52.8	287 bp	1.0	42.0
psaJ-rpl33_p2	ACTGCACCTGTTTTAGCTACA	TGAGCACAATTAATACACTCT	42.9	33.3	57.5	52.6	275 bp	1.0	42.0
psaJ-rpl33_p3	ACTGCACCTGTTTTAGCTACA	TTCATCATTTTGAGCACAATT	42.9	28.6	57.5	52.6	285 bp	1.0	42.0
psaJ-rpl33_p4	ACTGCACCTGTTTTAGCTACA	TTTTTCATCATTTTGAGCACA	42.9	28.6	57.5	52.8	288 bp	1.0	41.9
psaJ-rpl33_p5	ACTGCACCTGTTTTAGCTACA	TTTTTCATCATTTTGAGCACAA	42.9	27.3	57.5	53.7	288 bp	1.0	41.9