Hotspot

trnfM-CAU-rps14

Genome · 42970-43069 · 100 bp

Back to Conocephalum

Pi 0.0729

MarkerSeek score 79.50

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: Genome
length_bp: 100
variable_sites: 7
pi: 0.0729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	TACCAGACTGCTCTACTCCG	CGTGAAATGGCTCATGCATG	55.0	50.0	58.0	58.2	172-176 bp	1.0	83.4
trnfM-CAU-rps14_p2	AAGGTTATGAGCCTTGCGAG	CGTGAAATGGCTCATGCATG	50.0	50.0	58.0	58.2	193-197 bp	1.0	83.0
trnfM-CAU-rps14_p3	GGATTCGAACCTGTGACCTC	TTGCCTGGAGTCACGAAATC	55.0	50.0	58.0	58.2	189-193 bp	1.0	83.0
trnfM-CAU-rps14_p4	GGATTCGAACCTGTGACCTC	CGTGAAATGGCTCATGCATG	55.0	50.0	58.0	58.2	213-217 bp	1.0	82.7
trnfM-CAU-rps14_p5	GGATTCGAACCTGTGACCTC	CTTCGTGAAATGGCTCATGC	55.0	50.0	58.0	57.8	216-220 bp	1.0	82.5