Hotspot

psbZ-trnG-UCC

Genome · 42646-42769 · 124 bp

Back to Conocephalum

Pi 0.169

MarkerSeek score 80.50

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: Genome
length_bp: 124
variable_sites: 21
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTCCTGAAGGTTGGTCAAGT	GGGAATCGAACCCGCATATT	50.0	50.0	57.1	58.1	289-291 bp	1.0	77.4
psbZ-trnG-UCC_p2	CTCCTGAAGGTTGGTCAAGT	CGAACCCGCATATTCTCCTT	50.0	50.0	57.1	57.8	283-285 bp	1.0	76.5
psbZ-trnG-UCC_p3	CTCCTGAAGGTTGGTCAAGT	CCGCATATTCTCCTTGGCAA	50.0	50.0	57.1	58.3	278-280 bp	1.0	75.9
psbZ-trnG-UCC_p4	CTCCTGAAGGTTGGTCAAGT	AACCCGCATATTCTCCTTGG	50.0	50.0	57.1	57.7	281-283 bp	1.0	75.7
psbZ-trnG-UCC_p5	TCTCCTGAAGGTTGGTCAAG	GGGAATCGAACCCGCATATT	50.0	50.0	56.8	58.1	290-292 bp	1.0	75.1