Hotspot

trnH-GUG-ycf2

Genome · 30134-30468 · 335 bp

Back to Conocephalum

Pi 0.117

MarkerSeek score 80.00

Primer available yes

Label trnH-GUG-ycf2

Feature Metadata

label_name: trnH-GUG-ycf2
feature_type: igs
region: Genome
length_bp: 335
variable_sites: 39
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-ycf2_p1	AAGGCAGTGGATTGTGGATC	AACTCCATTGTGTCCATGGA	50.0	45.0	57.9	56.7	491-494 bp	1.0	75.4
trnH-GUG-ycf2_p2	GCCAAGTGGATTAAGGCAGT	AACTCCATTGTGTCCATGGA	50.0	45.0	58.2	56.7	503-506 bp	1.0	75.0
trnH-GUG-ycf2_p3	GATTGTGGATCCTCTACGCG	AACTCCATTGTGTCCATGGA	55.0	45.0	58.2	56.7	482-485 bp	1.0	74.8
trnH-GUG-ycf2_p4	AAGGCAGTGGATTGTGGATC	ACTCCATTGTGTCCATGGAT	50.0	45.0	57.9	56.5	490-493 bp	1.0	73.5
trnH-GUG-ycf2_p5	AAGGCAGTGGATTGTGGATC	AAACTCCATTGTGTCCATGGA	50.0	42.9	57.9	57.5	492-495 bp	1.0	73.4