Hotspot

ndhA

SSC · 121847-124009 · 2163 bp

Back to Conioselinum

Pi 0.00403

MarkerSeek score 50.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2163
variable_sites: 24
pi: 0.00403
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTAAGACAAGTCGTGAAGA	CACCCGGTTTTATCAATTTGCA	36.4	40.9	55.7	58.1	2322-2332 bp	1.0	41.2
ndhA_p2	TGTTTAAGACAAGTCGTGAAGA	ACCCGGTTTTATCAATTTGCA	36.4	38.1	55.7	56.7	2321-2331 bp	1.0	41.2
ndhA_p3	TTGTTTAAGACAAGTCGTGAAGA	CACCCGGTTTTATCAATTTGCA	34.8	40.9	56.4	58.1	2323-2333 bp	1.0	41.2
ndhA_p4	TGTTTAAGACAAGTCGTGAAGA	ACCACCCGGTTTTATCAATTTG	36.4	40.9	55.7	57.5	2324-2334 bp	1.0	41.2
ndhA_p5	AGACAAGTCGTGAAGATAGA	CACCCGGTTTTATCAATTTGCA	40.0	40.9	53.2	58.1	2316-2326 bp	1.0	41.2