Hotspot

ccsA-ndhD

SSC · 117466-117688 · 223 bp

Back to Conioselinum

Pi 0.0344

MarkerSeek score 58.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 223
variable_sites: 21
pi: 0.0344
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGCGTCTATGGGCTTTCTTA	CAGTTGACAAGGTCGAGGTT	50.0	50.0	57.4	57.8	373-380 bp	1.0	77.6
ccsA-ndhD_p2	GGCGTCTATGGGCTTTCTTA	AGTTGACAAGGTCGAGGTTC	50.0	50.0	57.4	57.5	372-379 bp	1.0	75.4
ccsA-ndhD_p3	GGCGTCTATGGGCTTTCTTA	GTTGACAAGGTCGAGGTTCT	50.0	50.0	57.4	57.5	371-378 bp	1.0	75.4
ccsA-ndhD_p4	GGCGTCTATGGGCTTTCTTA	TCAGTTGACAAGGTCGAGGT	50.0	50.0	57.4	58.6	374-381 bp	1.0	74.8
ccsA-ndhD_p5	GGCGTCTATGGGCTTTCTTA	ATCAGTTGACAAGGTCGAGG	50.0	50.0	57.4	57.2	375-382 bp	1.0	73.5