Hotspot

trnE-UUC-trnT-GGU

LSC · 32351-33120 · 770 bp

Back to Conioselinum

Pi 0.0096

MarkerSeek score 51.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 22
pi: 0.0096
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	824-854 bp	1.0	82.4
trnE-UUC-trnT-GGU_p2	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.2	57.9	859-889 bp	1.0	81.8
trnE-UUC-trnT-GGU_p3	AGTGTAGTACCCTACCCCCA	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.6	57.9	860-890 bp	1.0	78.7
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TGACTTACGCCTTACCATGG	50.0	50.0	57.8	57.3	852-882 bp	1.0	77.6
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	GCCTTACCATGGCGTTACTC	50.0	55.0	57.8	58.7	844-874 bp	1.0	77.5