Hotspot

rpoB-trnC-GCA

LSC · 27888-29105 · 1218 bp

Back to Conioselinum

Pi 0.0052

MarkerSeek score 55.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 21
pi: 0.0052
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1122-1372 bp	1.0	83.7
rpoB-trnC-GCA_p2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1123-1373 bp	1.0	83.7
rpoB-trnC-GCA_p3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1140-1390 bp	1.0	81.8
rpoB-trnC-GCA_p4	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1141-1391 bp	1.0	81.7
rpoB-trnC-GCA_p5	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1104-1354 bp	1.0	81.6