Hotspot

rps16-trnQ-UUG

LSC · 6824-8070 · 1247 bp

Back to Conioselinum

Pi 0.00214
MarkerSeek score 53.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1247
variable_sites
8
pi
0.00214
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 857-1366 bp 1.0 82.6
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 858-1367 bp 1.0 82.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 868-1377 bp 1.0 81.8
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 883-1392 bp 1.0 80.0
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 882-1391 bp 1.0 80.0