Hotspot

trnK-UUU-rps16

LSC · 5018-5733 · 716 bp

Back to Conioselinum

Pi 0.00884

MarkerSeek score 51.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 18
pi: 0.00884
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.0	56.5	835-842 bp	1.0	70.4
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	791-798 bp	1.0	69.6
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	790-797 bp	1.0	69.6
trnK-UUU-rps16_p4	CCCTCGCTTCATCCAAATGT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	834-841 bp	1.0	68.8
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.3	56.5	806-813 bp	1.0	68.7