Hotspot

rpl32-trnP-GGG

Genome · 110761-111284 · 524 bp

Back to Coniogramme

Pi 0.0827
MarkerSeek score 76.70
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
Genome
length_bp
524
variable_sites
43
pi
0.0827
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 CGGATCTTACACAGCTGCTT CTCTGGCACTGCTTCCTAAG 50.0 55.0 58.0 58.0 1422-1428 bp 1.0 86.1
rpl32-trnP-GGG_p2 CGGATCTTACACAGCTGCTT CTGGCACTGCTTCCTAAGAG 50.0 55.0 58.0 58.0 1420-1426 bp 1.0 86.1
rpl32-trnP-GGG_p3 CGGATCTTACACAGCTGCTT GTGGGTTAGGGAAGAGAGGT 50.0 55.0 58.0 58.0 1270-1276 bp 1.0 86.1
rpl32-trnP-GGG_p4 CGGATCTTACACAGCTGCTT TGTGGGTTAGGGAAGAGAGG 50.0 55.0 58.0 58.0 1271-1277 bp 1.0 86.1
rpl32-trnP-GGG_p5 CGGATCTTACACAGCTGCTT CAGCGTGTCTACCCATTTCA 50.0 50.0 58.0 57.9 1400-1406 bp 1.0 85.6