Hotspot

rpl14-rpl16

Genome · 77408-77873 · 466 bp

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Pi 0.0786

MarkerSeek score 77.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 466
variable_sites: 36
pi: 0.0786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCCGCTATTGTCTGCTACAT	CACCCGACTAGTGATTTCCG	50.0	55.0	57.5	58.1	542-546 bp	1.0	81.0
rpl14-rpl16_p2	CCCGCTATTGTCTGCTACAT	ACCCGACTAGTGATTTCCGA	50.0	50.0	57.5	58.2	541-545 bp	1.0	80.4
rpl14-rpl16_p3	CCCGCTATTGTCTGCTACAT	TAGCCACCCGACTAGTGATT	50.0	50.0	57.5	57.8	546-550 bp	1.0	80.3
rpl14-rpl16_p4	CCCGCTATTGTCTGCTACAT	CAAGATGCCTATAGCCACCC	50.0	55.0	57.5	57.8	557-561 bp	1.0	79.9
rpl14-rpl16_p5	CCCGCTATTGTCTGCTACAT	CCTATAGCCACCCGACTAGT	50.0	55.0	57.5	57.7	550-554 bp	1.0	79.2