Hotspot

trnD-GUC-trnY-GUA

Genome · 27421-27525 · 105 bp

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Pi 0.0762

MarkerSeek score 77.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 105
variable_sites: 8
pi: 0.0762
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TCAATTGGTTAGAGCACCGC	CCTACGCTGGTTCGAATCC	50.0	57.9	58.6	58.0	198 bp	1.0	70.6
trnD-GUC-trnY-GUA_p2	TCAATTGGTTAGAGCACCGC	ATGGGGACGGACTGTAAATC	50.0	50.0	58.6	57.0	229 bp	1.0	70.4
trnD-GUC-trnY-GUA_p3	TCAATTGGTTAGAGCACCGC	TGGGGACGGACTGTAAATCC	50.0	55.0	58.6	59.1	228 bp	1.0	69.7
trnD-GUC-trnY-GUA_p4	TCAATTGGTTAGAGCACCGC	CTGGTTCGAATCCAGCTCG	50.0	57.9	58.6	58.3	192 bp	1.0	68.3
trnD-GUC-trnY-GUA_p5	TCAATTGGTTAGAGCACCGC	AATGGGGACGGACTGTAAATC	50.0	47.6	58.6	57.7	230 bp	1.0	68.2