Hotspot

ycf1

SSC · 128105-132259 · 4155 bp

Back to Commiphora

Pi 0.0111

MarkerSeek score 52.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4155
variable_sites: 108
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGAATTCCATCATCCACCG	CTCTCGTGCATGGACCTTTT	50.0	50.0	58.0	58.2	350-456 bp	1.0	82.0
ycf1_p2	ACGAATTCCATCATCCACCG	TCTCGTGCATGGACCTTTTC	50.0	50.0	58.0	58.2	349-455 bp	1.0	82.0
ycf1_p3	TGCGGGCGATATTTTTAGCT	CTCTCGTGCATGGACCTTTT	45.0	50.0	58.0	58.2	855-964 bp	1.0	81.7
ycf1_p4	GCATCGAGCCTTTGATGAGA	CTCTCGTGCATGGACCTTTT	50.0	50.0	58.1	58.2	510-616 bp	1.0	81.7
ycf1_p5	TGCGGGCGATATTTTTAGCT	TCTCGTGCATGGACCTTTTC	45.0	50.0	58.0	58.2	854-963 bp	1.0	81.6