Hotspot

psbC

LSC · 35744-37165 · 1422 bp

Back to Commiphora

Pi 0.011

MarkerSeek score 46.00

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 33
pi: 0.011
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AGGGTATTCGTGCTTGGATG	TAAGCATTGGATCCCCTGTC	50.0	50.0	57.7	57.0	1481-1482 bp	1.0	70.9
psbC_p2	AGGGTATTCGTGCTTGGATG	ACATAAGCATTGGATCCCCTG	50.0	47.6	57.7	57.8	1484-1485 bp	1.0	69.2
psbC_p3	AGGGTATTCGTGCTTGGATG	CATAAGCATTGGATCCCCTGT	50.0	47.6	57.7	57.8	1483-1484 bp	1.0	69.2
psbC_p4	GAGGGTATTCGTGCTTGGAT	TAAGCATTGGATCCCCTGTC	50.0	50.0	57.4	57.0	1482-1483 bp	1.0	68.6
psbC_p5	GGGTATTCGTGCTTGGATGG	TAAGCATTGGATCCCCTGTC	55.0	50.0	58.7	57.0	1480-1481 bp	1.0	68.0