Hotspot

ndhC-trnV-UAC

LSC · 53259-54200 · 942 bp

Back to Commiphora

Pi 0.00743
MarkerSeek score 66.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
942
variable_sites
14
pi
0.00743
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGCCCTTTGCTAATTGG GCACGTTGGGTCTTTGAAAG 50.0 50.0 58.1 57.9 1138-1161 bp 1.0 81.3
ndhC-trnV-UAC_p2 TCTGGCCCTTTGCTAATTGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1074-1097 bp 1.0 80.9
ndhC-trnV-UAC_p3 TCTGGCCCTTTGCTAATTGG AATGCACGTTGGGTCTTTGA 50.0 45.0 58.1 58.3 1141-1164 bp 1.0 79.8
ndhC-trnV-UAC_p4 TCTGGCCCTTTGCTAATTGG ATGCACGTTGGGTCTTTGAA 50.0 45.0 58.1 58.3 1140-1163 bp 1.0 79.8
ndhC-trnV-UAC_p5 CCCTTTGCTAATTGGGGCTA GCACGTTGGGTCTTTGAAAG 50.0 50.0 57.5 57.9 1133-1156 bp 1.0 78.9