Hotspot

petN-psbM

LSC · 30323-30922 · 600 bp

Back to Commiphora

Pi 0.0312

MarkerSeek score 67.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 42
pi: 0.0312
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GCAAAGGTCTTGGGTCAGAA	ATGGTTGTACAAGGAAGGCG	50.0	50.0	58.0	58.2	1260-1567 bp	1.0	81.4
petN-psbM_p2	TTCAGCAAAGGTCTTGGGTC	ATGGTTGTACAAGGAAGGCG	50.0	50.0	58.0	58.2	1264-1571 bp	1.0	81.4
petN-psbM_p3	GACGTGACTGCGTATCTTGT	ATGGTTGTACAAGGAAGGCG	50.0	50.0	58.0	58.2	1349-1656 bp	1.0	81.2
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	ATGGTTGTACAAGGAAGGCG	50.0	50.0	58.2	58.2	1138-1444 bp	1.0	80.6
petN-psbM_p5	TGAAAGTCTCTGAGTGCACG	ATGGTTGTACAAGGAAGGCG	50.0	50.0	57.9	58.2	1522-1830 bp	1.0	80.0