Hotspot

trnK-UUU-rps16

LSC · 4474-5533 · 1060 bp

Back to Commiphora

Pi 0.00717
MarkerSeek score 64.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1060
variable_sites
15
pi
0.00717
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCGATGGTATGGACGAATC ATCTTTCCTTGAAAGGGGCG 55.0 50.0 57.9 58.2 1257-1336 bp 1.0 79.5
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC ATCTTTCCTTGAAAGGGGCG 55.0 50.0 57.9 58.2 1256-1335 bp 1.0 79.5
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT ATCTTTCCTTGAAAGGGGCG 50.0 50.0 58.2 58.2 1195-1274 bp 1.0 78.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT ATCTTTCCTTGAAAGGGGCG 50.0 50.0 58.2 58.2 1194-1273 bp 1.0 78.9
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA ATCTTTCCTTGAAAGGGGCG 50.0 50.0 58.3 58.2 1204-1283 bp 1.0 78.0