primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 20 20 55.0 55.0 58.1 57.9 0.0 0.0 0.203648 130 548 479-487 1.000000 83.5 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.208010 130 548 493-501 1.000000 83.3 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.209518 130 548 492-500 1.000000 83.3 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.209518 130 548 494-502 1.000000 83.3 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 ATGGTAGTTATGGGCGAACG TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.8 57.9 0.0 0.0 0.309028 130 548 544-552 1.000000 82.1 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCCGATGGTATGGACGAATC ATCTTTCCTTGAAAGGGGCG 20 20 55.0 50.0 57.9 58.2 0.0 14.6 0.240862 4474 5533 1257-1336 1.000000 79.5 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CCGATGGTATGGACGAATCC ATCTTTCCTTGAAAGGGGCG 20 20 55.0 50.0 57.9 58.2 0.0 14.6 0.240862 4474 5533 1256-1335 1.000000 79.5 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 AAAGCCGAGTACTCTACCGT ATCTTTCCTTGAAAGGGGCG 20 20 50.0 50.0 58.2 58.2 20.3 14.6 0.328996 4474 5533 1195-1274 1.000000 78.9 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 AAGCCGAGTACTCTACCGTT ATCTTTCCTTGAAAGGGGCG 20 20 50.0 50.0 58.2 58.2 20.3 14.6 0.328996 4474 5533 1194-1273 1.000000 78.9 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 CCGCACTTAAAAGCCGAGTA ATCTTTCCTTGAAAGGGGCG 20 20 50.0 50.0 58.3 58.2 5.3 14.6 0.443383 4474 5533 1204-1283 1.000000 78.0 atpF-atpH_p1 atpF-atpH 1 CCAGTGAACCAAGGAAACGA CCGAGGCAGAGGGAAAAATA 20 20 50.0 50.0 58.0 57.3 0.0 0.0 0.748302 14523 15058 677-704 1.000000 78.3 atpF-atpH_p2 atpF-atpH 2 CCAGTGAACCAAGGAAACGA TGGACTGGTTGTAGCATTAGC 20 21 50.0 47.6 58.0 58.0 0.0 0.0 1.052311 14523 15058 607-634 1.000000 76.3 atpF-atpH_p3 atpF-atpH 3 CCAGTGAACCAAGGAAACGA GCAGAGGGAAAAATACGAGGT 20 21 50.0 47.6 58.0 58.0 0.0 0.0 1.050974 14523 15058 672-699 1.000000 75.9 atpF-atpH_p4 atpF-atpH 4 CAAATGGCCAGTGAACCAAG CCGAGGCAGAGGGAAAAATA 20 20 50.0 50.0 57.6 57.3 13.0 0.0 1.153983 14523 15058 684-711 1.000000 75.0 atpF-atpH_p5 atpF-atpH 5 CCAGTGAACCAAGGAAACGA GAGGCAGAGGGAAAAATACGA 20 21 50.0 47.6 58.0 57.7 0.0 0.0 1.300187 14523 15058 675-702 1.000000 73.9 rpoB_p1 rpoB 1 GATACCGGGGCTTTGCAATA TCACTCACGTATTCTTCCGA 20 20 50.0 45.0 58.0 56.0 6.3 0.0 1.988692 27837 27956 180-180 1.000000 69.0 rpoB_p2 rpoB 2 CGAAAGCCCATTATGGTCCA TCACTCACGTATTCTTCCGA 20 20 50.0 45.0 57.9 56.0 10.6 0.0 2.015687 27837 27956 216-216 1.000000 67.9 rpoB_p3 rpoB 3 ACCGAAAGCCCATTATGGTC TCACTCACGTATTCTTCCGA 20 20 50.0 45.0 57.9 56.0 10.6 0.0 2.017134 27837 27956 218-218 1.000000 67.9 rpoB_p4 rpoB 4 GATACCGGGGCTTTGCAATA TCACTCACGTATTCTTCCGAA 20 21 50.0 42.9 58.0 56.8 6.3 0.0 2.226741 27837 27956 180-180 1.000000 67.1 rpoB_p5 rpoB 5 AATAGATACCGGGGCTTTGC TCACTCACGTATTCTTCCGA 20 20 50.0 45.0 57.7 56.0 6.3 0.0 2.236296 27837 27956 184-184 1.000000 66.9 petN-psbM_p1 petN-psbM 1 GCAAAGGTCTTGGGTCAGAA ATGGTTGTACAAGGAAGGCG 20 20 50.0 50.0 58.0 58.2 0.0 9.0 0.197527 30323 30922 1260-1567 1.000000 81.4 petN-psbM_p2 petN-psbM 2 TTCAGCAAAGGTCTTGGGTC ATGGTTGTACAAGGAAGGCG 20 20 50.0 50.0 58.0 58.2 0.0 9.0 0.197527 30323 30922 1264-1571 1.000000 81.4 petN-psbM_p3 petN-psbM 3 GACGTGACTGCGTATCTTGT ATGGTTGTACAAGGAAGGCG 20 20 50.0 50.0 58.0 58.2 0.0 9.0 0.200242 30323 30922 1349-1656 1.000000 81.2 petN-psbM_p4 petN-psbM 4 TAGTAAGTCTCGCTTGGGCT ATGGTTGTACAAGGAAGGCG 20 20 50.0 50.0 58.2 58.2 0.0 9.0 0.330132 30323 30922 1138-1444 1.000000 80.6 petN-psbM_p5 petN-psbM 5 TGAAAGTCTCTGAGTGCACG ATGGTTGTACAAGGAAGGCG 20 20 50.0 50.0 57.9 58.2 12.0 9.0 0.318848 30323 30922 1522-1830 1.000000 80.0 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 CGGTGCTCTGACCAATTGAA TTTTCTTCCTGGGTCGATGC 20 20 50.0 50.0 58.5 58.2 7.1 0.0 0.656115 31633 32126 574-618 1.000000 79.9 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.716274 31633 32126 520-564 1.000000 79.6 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.717825 31633 32126 519-563 1.000000 79.6 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 20 20 50.0 55.0 58.5 58.4 7.1 0.0 0.828828 31633 32126 541-585 1.000000 78.6 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 20 20 50.0 50.0 58.5 57.6 7.1 0.0 0.852505 31633 32126 540-584 1.000000 78.4 psbC_p1 psbC 1 AGGGTATTCGTGCTTGGATG TAAGCATTGGATCCCCTGTC 20 20 50.0 50.0 57.7 57.0 0.0 9.9 1.361701 35744 37165 1481-1482 1.000000 70.9 psbC_p2 psbC 2 AGGGTATTCGTGCTTGGATG ACATAAGCATTGGATCCCCTG 20 21 50.0 47.6 57.7 57.8 0.0 9.9 1.566297 35744 37165 1484-1485 1.000000 69.2 psbC_p3 psbC 3 AGGGTATTCGTGCTTGGATG CATAAGCATTGGATCCCCTGT 20 21 50.0 47.6 57.7 57.8 0.0 9.9 1.566297 35744 37165 1483-1484 1.000000 69.2 psbC_p4 psbC 4 GAGGGTATTCGTGCTTGGAT TAAGCATTGGATCCCCTGTC 20 20 50.0 50.0 57.4 57.0 0.0 9.9 1.651701 35744 37165 1482-1483 1.000000 68.6 psbC_p5 psbC 5 GGGTATTCGTGCTTGGATGG TAAGCATTGGATCCCCTGTC 20 20 55.0 50.0 58.7 57.0 0.0 9.9 1.720640 35744 37165 1480-1481 1.000000 68.0 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 48925 49826 920-1072 1.000000 84.0 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 48925 49826 928-1080 1.000000 83.4 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 48925 49826 952-1104 1.000000 83.1 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 48925 49826 960-1112 1.000000 82.5 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 48925 49826 934-1086 1.000000 82.1 ndhK-ndhC_p1 ndhK-ndhC 1 CTGGAGAGCCTTGACCAATT AAAGGAGCATTGGAGTGGTC 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.710471 52835 52895 180-184 1.000000 77.4 ndhK-ndhC_p2 ndhK-ndhC 2 ACTGGAGAGCCTTGACCAAT AAAGGAGCATTGGAGTGGTC 20 20 50.0 50.0 58.6 57.8 0.0 0.0 0.840258 52835 52895 181-185 1.000000 76.3 ndhK-ndhC_p3 ndhK-ndhC 3 CTGGAGAGCCTTGACCAATT CAGTTTATGCGTGGCGAAAA 20 20 50.0 45.0 57.5 57.7 0.0 0.0 0.827834 52835 52895 197-201 1.000000 76.2 ndhK-ndhC_p4 ndhK-ndhC 4 CTGGAGAGCCTTGACCAATT GTTTATGCGTGGCGAAAAGG 20 20 50.0 50.0 57.5 58.4 0.0 0.0 0.890625 52835 52895 195-199 1.000000 75.7 ndhK-ndhC_p5 ndhK-ndhC 5 ACTGGAGAGCCTTGACCAAT CAGTTTATGCGTGGCGAAAA 20 20 50.0 45.0 58.6 57.7 0.0 0.0 0.957621 52835 52895 198-202 1.000000 75.1 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCTGGCCCTTTGCTAATTGG GCACGTTGGGTCTTTGAAAG 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.268368 53259 54200 1138-1161 1.000000 81.3 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCTGGCCCTTTGCTAATTGG AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.345979 53259 54200 1074-1097 1.000000 80.9 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 TCTGGCCCTTTGCTAATTGG AATGCACGTTGGGTCTTTGA 20 20 50.0 45.0 58.1 58.3 0.0 0.0 0.462341 53259 54200 1141-1164 1.000000 79.8 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 TCTGGCCCTTTGCTAATTGG ATGCACGTTGGGTCTTTGAA 20 20 50.0 45.0 58.1 58.3 0.0 0.0 0.462341 53259 54200 1140-1163 1.000000 79.8 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CCCTTTGCTAATTGGGGCTA GCACGTTGGGTCTTTGAAAG 20 20 50.0 50.0 57.5 57.9 0.0 0.0 0.572272 53259 54200 1133-1156 1.000000 78.9 petA-psbJ_p1 petA-psbJ 1 ATCCATTACGTGTCCAAGGC TATTCCGGATTGGGTTCGTC 20 20 50.0 50.0 58.0 57.4 0.0 4.1 0.646018 65846 66965 957-1269 1.000000 80.0 petA-psbJ_p2 petA-psbJ 2 CATTACGTGTCCAAGGCCTT TATTCCGGATTGGGTTCGTC 20 20 50.0 50.0 58.2 57.4 13.8 4.1 0.786415 65846 66965 954-1266 1.000000 78.8 petA-psbJ_p3 petA-psbJ 3 ATCCATTACGTGTCCAAGGC GGATTGGGTTCGTCCTTGTA 20 20 50.0 50.0 58.0 57.2 0.0 0.3 0.812380 65846 66965 951-1263 1.000000 78.6 petA-psbJ_p4 petA-psbJ 4 CATTACGTGTCCAAGGCCTT GGATTGGGTTCGTCCTTGTA 20 20 50.0 50.0 58.2 57.2 13.8 0.3 0.952776 65846 66965 948-1260 1.000000 77.5 petA-psbJ_p5 petA-psbJ 5 TTACGTGTCCAAGGCCTTTT TATTCCGGATTGGGTTCGTC 20 20 45.0 50.0 57.6 57.4 17.6 4.1 0.959337 65846 66965 952-1264 1.000000 77.5 trnN-GUU-ndhF_p1 trnN-GUU-ndhF 1 GAACTTGGGTGTGGGTCTTT ATATGTAGGGGGAAGTCGCA 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.164475 112878 114333 2120-2159 1.000000 83.3 trnN-GUU-ndhF_p2 trnN-GUU-ndhF 2 GAACTTGGGTGTGGGTCTTT TATGTAGGGGGAAGTCGCAT 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.164475 112878 114333 2119-2158 1.000000 83.3 trnN-GUU-ndhF_p3 trnN-GUU-ndhF 3 GAACTTGGGTGTGGGTCTTT GGGGAAGTCGCATCTCTTTT 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.182708 112878 114333 2112-2151 1.000000 83.2 trnN-GUU-ndhF_p4 trnN-GUU-ndhF 4 CTATGGAACTTGGGTGTGGG TATGTAGGGGGAAGTCGCAT 20 20 55.0 50.0 57.9 57.9 0.0 0.0 0.225881 112878 114333 2124-2163 1.000000 82.8 trnN-GUU-ndhF_p5 trnN-GUU-ndhF 5 GAACTTGGGTGTGGGTCTTT TCCTTTGTTTGTTGGAGCCA 20 20 50.0 45.0 57.9 57.8 0.0 0.0 0.218868 112878 114333 2589-2628 1.000000 82.7 ccsA_p1 ccsA 1 TCCATTCCGTATACTTGAGGAAC CAGGCCCTTTCCTTTCTTCA 23 20 43.5 50.0 57.7 57.7 10.9 0.0 3.565111 118480 119448 1045-1046 1.000000 52.9 ccsA_p2 ccsA 2 CCATTCCGTATACTTGAGGAACT CAGGCCCTTTCCTTTCTTCA 23 20 43.5 50.0 57.7 57.7 10.9 0.0 3.570686 118480 119448 1044-1045 1.000000 52.8 ccsA_p3 ccsA 3 TCCATTCCGTATACTTGAGGA CAGGCCCTTTCCTTTCTTCA 21 20 42.9 50.0 55.6 57.7 0.6 0.0 3.702125 118480 119448 1045-1046 1.000000 51.8 ccsA_p4 ccsA 4 TCCATTCCGTATACTTGAGGAAC TCATCAGGCCCTTTCCTTTC 23 20 43.5 50.0 57.7 57.5 10.9 0.0 3.786741 118480 119448 1049-1050 1.000000 51.1 ccsA_p5 ccsA 5 CCATTCCGTATACTTGAGGAACT TCATCAGGCCCTTTCCTTTC 23 20 43.5 50.0 57.7 57.5 10.9 0.0 3.792316 118480 119448 1048-1049 1.000000 51.0 ccsA-ndhD_p1 ccsA-ndhD 1 TGATGGTAGTAGGCTACGCA TTATCGATGGCTTCCCTTGC 20 20 50.0 50.0 57.9 58.0 18.4 8.5 0.094191 119449 119764 1214-1227 1.000000 82.3 ccsA-ndhD_p2 ccsA-ndhD 2 TGATGGTAGTAGGCTACGCA GCTGGCTATCCCAATTCCAA 20 20 50.0 50.0 57.9 57.9 18.4 0.0 0.133287 119449 119764 1257-1270 1.000000 81.9 ccsA-ndhD_p3 ccsA-ndhD 3 TGATGGTAGTAGGCTACGCA GCATATGGGTTGGTTCGGAT 20 20 50.0 50.0 57.9 58.0 18.4 4.6 0.080253 119449 119764 1584-1597 1.000000 81.8 ccsA-ndhD_p4 ccsA-ndhD 4 TGATGGTAGTAGGCTACGCA TACAGGACTTAACGGAGCCA 20 20 50.0 50.0 57.9 58.1 18.4 0.0 0.134022 119449 119764 1381-1394 1.000000 81.7 ccsA-ndhD_p5 ccsA-ndhD 5 TGTGGCTTCTATGGGCTTTC GCATATGGGTTGGTTCGGAT 20 20 50.0 50.0 57.9 58.0 0.0 4.6 0.147514 119449 119764 1133-1146 1.000000 81.3 ndhD-psaC_p1 ndhD-psaC 1 TTAAAGAACCCGCGGCTATC AAACAACTCGAAGCATGGGT 20 20 50.0 45.0 58.1 58.0 22.8 0.0 0.074712 121286 121434 259-261 1.000000 85.9 ndhD-psaC_p2 ndhD-psaC 2 TTAAAGAACCCGCGGCTATC GGCATGAAACAACTCGAAGC 20 20 50.0 50.0 58.1 58.0 22.8 0.0 0.086865 121286 121434 265-267 1.000000 85.6 ndhD-psaC_p3 ndhD-psaC 3 AAAATTAAAGAACCCGCGGC AAACAACTCGAAGCATGGGT 20 20 45.0 45.0 57.6 58.0 22.8 0.0 0.376070 121286 121434 263-265 1.000000 83.4 ndhD-psaC_p4 ndhD-psaC 4 AAAATTAAAGAACCCGCGGC GGCATGAAACAACTCGAAGC 20 20 45.0 50.0 57.6 58.0 22.8 0.0 0.388224 121286 121434 269-271 1.000000 83.1 ndhD-psaC_p5 ndhD-psaC 5 TTAAAGAACCCGCGGCTATC AAGAGATGTGAATCCGCCTG 20 20 50.0 50.0 58.1 57.7 22.8 0.0 0.378324 121286 121434 320-322 1.000000 82.2 ndhH_p1 ndhH 1 GACAAGCATCCATATGATCCCA TGAACTTCTTTTCGTTTTCAACA 22 23 45.5 30.4 58.0 55.8 3.7 0.0 7.261431 126178 127359 1330-1331 1.000000 43.2 ndhH_p2 ndhH 2 AGGGACAAGCATCCATATGA TGAACTTCTTTTCGTTTTCAACA 20 23 45.0 30.4 56.0 55.8 3.7 0.0 7.268431 126178 127359 1333-1334 1.000000 43.2 ndhH_p3 ndhH 3 GACAAGCATCCATATGATCCCA TCATGTAAGAATGAATCGAGGA 22 22 45.5 36.4 58.0 54.7 3.7 0.0 7.291429 126178 127359 1305-1306 1.000000 43.2 ndhH_p4 ndhH 4 AGGGACAAGCATCCATATGA TCATGTAAGAATGAATCGAGGA 20 22 45.0 36.4 56.0 54.7 3.7 0.0 7.298429 126178 127359 1308-1309 1.000000 43.2 ndhH_p5 ndhH 5 ACAAGCATCCATATGATCCCA TGAACTTCTTTTCGTTTTCAACA 21 23 42.9 30.4 56.8 55.8 3.7 0.0 7.408858 126178 127359 1329-1330 1.000000 43.2 ycf1_p1 ycf1 1 ACGAATTCCATCATCCACCG CTCTCGTGCATGGACCTTTT 20 20 50.0 50.0 58.0 58.2 0.0 12.1 0.232928 131769 131918 350-456 1.000000 82.0 ycf1_p2 ycf1 2 ACGAATTCCATCATCCACCG TCTCGTGCATGGACCTTTTC 20 20 50.0 50.0 58.0 58.2 0.0 12.1 0.238030 131769 131918 349-455 1.000000 82.0 ycf1_p3 ycf1 3 TGCGGGCGATATTTTTAGCT CTCTCGTGCATGGACCTTTT 20 20 45.0 50.0 58.0 58.2 0.0 12.1 0.224693 131769 131918 855-964 1.000000 81.7 ycf1_p4 ycf1 4 GCATCGAGCCTTTGATGAGA CTCTCGTGCATGGACCTTTT 20 20 50.0 50.0 58.1 58.2 10.9 12.1 0.241099 131769 131918 510-616 1.000000 81.7 ycf1_p5 ycf1 5 TGCGGGCGATATTTTTAGCT TCTCGTGCATGGACCTTTTC 20 20 45.0 50.0 58.0 58.2 0.0 12.1 0.229796 131769 131918 854-963 1.000000 81.6 rrn16S_p1 rrn16S 1 AGTTTCGTAAGGGGACTGGA TGTACTCCACGGATAGAGGA 20 20 50.0 50.0 58.0 56.6 0.0 0.0 1.431359 144528 146027 1667-1672 1.000000 69.5 rrn16S_p2 rrn16S 2 ATGATGCCATGTGAATCGCT TGTACTCCACGGATAGAGGA 20 20 45.0 50.0 58.0 56.6 0.0 0.0 1.434496 144528 146027 1724-1729 1.000000 69.5 rrn16S_p3 rrn16S 3 GCAGGCTACCATGAGACAAA TGTACTCCACGGATAGAGGA 20 20 50.0 50.0 57.9 56.6 0.0 0.0 1.525412 144528 146027 1647-1652 1.000000 68.8 rrn16S_p4 rrn16S 4 AGTTTCGTAAGGGGACTGGA TGTACTCCACGGATAGAGGAA 20 21 50.0 47.6 58.0 57.3 0.0 0.0 1.677205 144528 146027 1667-1672 1.000000 67.5 rrn16S_p5 rrn16S 5 ATGATGCCATGTGAATCGCT TGTACTCCACGGATAGAGGAA 20 21 45.0 47.6 58.0 57.3 0.0 0.0 1.680342 144528 146027 1724-1729 1.000000 67.5