Hotspot

ycf3

LSC · 42307-44241 · 1935 bp

Back to Commidendrum

Pi 0.000258

MarkerSeek score 44.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1935
variable_sites: 1
pi: 0.000258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GCCCCAACAAACAATTAATT	GGATCATTTTCCCCCGAGAA	35.0	50.0	52.8	57.3	2109 bp	1.0	40.0
ycf3_p2	GCCCCAACAAACAATTAATT	TCCCCCGAGAAGTAATGAGA	35.0	50.0	52.8	57.2	2100 bp	1.0	40.0
ycf3_p3	GCCCCAACAAACAATTAATT	TCCCCCGAGAAGTAATGAGAA	35.0	47.6	52.8	57.9	2100 bp	1.0	40.0
ycf3_p4	GCCCCAACAAACAATTAATT	TTCCCCCGAGAAGTAATGAGA	35.0	47.6	52.8	57.9	2101 bp	1.0	40.0
ycf3_p5	GCCCCAACAAACAATTAATT	CCCCCGAGAAGTAATGAGAAC	35.0	52.4	52.8	57.8	2099 bp	1.0	40.0