Hotspot

trnT-GGU

LSC · 31153-31220 · 68 bp

Back to Commidendrum

Pi 0.00735
MarkerSeek score 43.60
Primer available yes
Label trnT-GGU

Feature Metadata

label_name
trnT-GGU
feature_type
tRNA
region
LSC
length_bp
68
variable_sites
1
pi
0.00735
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU_p1 GTGACCCGTTCAGGAATTCA TCTTCATATGAATACTACGACTGA 50.0 33.3 57.8 54.7 141 bp 1.0 40.6
trnT-GGU_p2 CAGTGACCCGTTCAGGAATT TCTTCATATGAATACTACGACTGA 50.0 33.3 57.8 54.7 143 bp 1.0 40.6
trnT-GGU_p3 TACAGTGACCCGTTCAGGAA TCTTCATATGAATACTACGACTGA 50.0 33.3 58.3 54.7 145 bp 1.0 40.6
trnT-GGU_p4 AGTGACCCGTTCAGGAATTC TCTTCATATGAATACTACGACTGA 50.0 33.3 57.5 54.7 142 bp 1.0 40.6
trnT-GGU_p5 GTGACCCGTTCAGGAATTCA TCTTCATATGAATACTACGACTGAA 50.0 32.0 57.8 55.4 141 bp 1.0 40.6