Hotspot

rps16

LSC · 4992-6139 · 1148 bp

Back to Commidendrum

Pi 0.000436

MarkerSeek score 45.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1148
variable_sites: 1
pi: 0.000436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGACCAAGAAAGGA	TCTCTGTACCGACTGAACCA	40.0	50.0	53.7	57.7	1325 bp	1.0	43.4
rps16_p2	ACGAATAGACCAAGAAAGGAA	TCTCTGTACCGACTGAACCA	38.1	50.0	54.6	57.7	1325 bp	1.0	42.5
rps16_p3	ACGAATAGACCAAGAAAGGAAA	TCTCTGTACCGACTGAACCA	36.4	50.0	55.4	57.7	1325 bp	1.0	40.9
rps16_p4	ACGAATAGACCAAGAAAGGA	GTCTCTGTACCGACTGAACC	40.0	55.0	53.7	57.4	1326 bp	1.0	40.5
rps16_p5	ACGAATAGACCAAGAAAGGAA	GTCTCTGTACCGACTGAACC	38.1	55.0	54.6	57.4	1326 bp	1.0	40.1