Hotspot

psbA

LSC · 274-1335 · 1062 bp

Back to Commidendrum

Pi 0.000628

MarkerSeek score 40.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAAAGGAGCAATAACGCCCT	TATTGGTTGACACGCGCATA	45.0	45.0	57.8	58.0	1278 bp	1.0	78.2
psbA_p2	AAAAGGAGCAATAACGCCCT	TGGTTGACACGCGCATATAA	45.0	45.0	57.8	58.0	1275 bp	1.0	78.2
psbA_p3	AAAAGGAGCAATAACGCCCT	TTGGTTGACACGCGCATATA	45.0	45.0	57.8	58.0	1276 bp	1.0	78.2
psbA_p4	GGAGCAATAACGCCCTCTTT	TGGTTGACACGCGCATATAA	50.0	45.0	58.2	58.0	1271 bp	1.0	78.0
psbA_p5	GGAGCAATAACGCCCTCTTT	TTGGTTGACACGCGCATATA	50.0	45.0	58.2	58.0	1272 bp	1.0	78.0