Hotspot

atpI

LSC · 24170-24913 · 744 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 GTCAAGTCGAGAAAGAGATGGT TTTTAGCCACGCACACATTG 45.5 45.0 57.8 57.6 871 bp 1.0 59.2
atpI_p2 GTCAAGTCGAGAAAGAGATGGT ACGCACACATTGTCTTAGGT 45.5 45.0 57.8 57.5 863 bp 1.0 58.2
atpI_p3 AGTCAAGTCGAGAAAGAGATGG TTTTAGCCACGCACACATTG 45.5 45.0 57.6 57.6 872 bp 1.0 57.1
atpI_p4 GTCAAGTCGAGAAAGAGATGGT TTTAGCCACGCACACATTGT 45.5 45.0 57.8 58.7 870 bp 1.0 57.1
atpI_p5 AGTCAAGTCGAGAAAGAGATGG ACGCACACATTGTCTTAGGT 45.5 45.0 57.6 57.5 864 bp 1.0 56.1