Hotspot

trnL-UAG-rpl32

SSC · 123223-124058 · 836 bp

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Pi 0.000598
MarkerSeek score 45.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
836
variable_sites
1
pi
0.000598
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GAAAGGATATTGGGCGTCGT 50.0 50.0 58.0 58.1 965 bp 1.0 79.6
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GAAAGGATATTGGGCGTCGT 50.0 50.0 58.0 58.1 966 bp 1.0 79.6
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GAAAGGATATTGGGCGTCGT 50.0 50.0 57.9 58.1 975 bp 1.0 78.7
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC GAAAGGATATTGGGCGTCGT 55.0 50.0 57.8 58.1 974 bp 1.0 78.1
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC GAAAGGATATTGGGCGTCGT 55.0 50.0 57.8 58.1 973 bp 1.0 78.1