Hotspot

rps8-rpl14

LSC · 80508-80697 · 190 bp

Back to Commidendrum

Pi 0

MarkerSeek score 48.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACTCGAACCACGCTTTTTCT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	58.1	58.0	340-353 bp	1.0	82.7
rps8-rpl14_p2	ACTCGAACCACGCTTTTTCT	CGATTGCCCGAGAATTGAGA	45.0	50.0	58.1	58.1	322-335 bp	1.0	82.5
rps8-rpl14_p3	ACTCGAACCACGCTTTTTCT	TTGCCCGAGAATTGAGACAG	45.0	50.0	58.1	57.9	319-332 bp	1.0	82.2
rps8-rpl14_p4	ACTCGAACCACGCTTTTTCT	GCCCGAGAATTGAGACAGTT	45.0	50.0	58.1	57.9	317-330 bp	1.0	82.2
rps8-rpl14_p5	ACTCGAACCACGCTTTTTCT	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	58.1	58.1	338-351 bp	1.0	81.9