Hotspot

trnR-UCU-trnG-UCC

LSC · 29732-29963 · 232 bp

Back to Commidendrum

Pi 0.00287
MarkerSeek score 36.00
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
232
variable_sites
1
pi
0.00287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 311-312 bp 1.0 63.9
trnR-UCU-trnG-UCC_p2 AGAAGACCTCTGTCCTATCCA CCTAGCCTTCCAAGCTAACG 47.6 55.0 56.9 58.1 314-315 bp 1.0 63.6
trnR-UCU-trnG-UCC_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 317-318 bp 1.0 63.2
trnR-UCU-trnG-UCC_p4 AGGTTTAGAAGACCTCTGTCCT CCTAGCCTTCCAAGCTAACG 45.5 55.0 57.9 58.1 320-321 bp 1.0 63.0
trnR-UCU-trnG-UCC_p5 AGAAGACCTCTGTCCTATCCA CCCTAGCCTTCCAAGCTAAC 47.6 55.0 56.9 57.7 315-316 bp 1.0 61.4