Hotspot

rpoC2-rps2

LSC · 22990-23220 · 231 bp

Back to Commidendrum

Pi 0.00216

MarkerSeek score 45.50

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 1
pi: 0.00216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TGCCTTTCAAAGAATTTCCATGA	CCATGGCCAAAATGAACTCC	34.8	50.0	57.2	57.3	345-347 bp	1.0	44.8
rpoC2-rps2_p2	TGCCTTTCAAAGAATTTCCATGA	GGCCAAAATGAACTCCTGCT	34.8	50.0	57.2	58.7	341-343 bp	1.0	44.3
rpoC2-rps2_p3	TCTTGCCTTTCAAAGAATTTCCA	CCATGGCCAAAATGAACTCC	34.8	50.0	57.1	57.3	348-350 bp	1.0	44.3
rpoC2-rps2_p4	TCTTGCCTTTCAAAGAATTTCCA	GGCCAAAATGAACTCCTGCT	34.8	50.0	57.1	58.7	344-346 bp	1.0	43.8
rpoC2-rps2_p5	TGCCTTTCAAAGAATTTCCATGA	TGGCCAAAATGAACTCCTGC	34.8	50.0	57.2	59.0	342-344 bp	1.0	42.0