Hotspot

ycf1

SSC · 129079-134436 · 5358 bp

Back to Commelina

Pi 0.0134

MarkerSeek score 51.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5358
variable_sites: 107
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATTTCCGTTTCTCCTCCCC	TGGTATTCAATTATCAGAAACAGA	55.0	29.2	58.0	54.0	190 bp	1.0	44.2
ycf1_p2	GGATTTCCGTTTCTCCTCCC	TGGTATTCAATTATCAGAAACAGA	55.0	29.2	58.0	54.0	191 bp	1.0	44.2
ycf1_p3	GGGATTTCCGTTTCTCCTCC	TGGTATTCAATTATCAGAAACAGA	55.0	29.2	58.0	54.0	192 bp	1.0	44.2
ycf1_p4	GGGGATTTCCGTTTCTCCTC	TGGTATTCAATTATCAGAAACAGA	55.0	29.2	58.0	54.0	193 bp	1.0	44.1
ycf1_p5	GAACCAAAGGGGATTTCCGT	TGGTATTCAATTATCAGAAACAGA	50.0	29.2	58.1	54.0	201 bp	1.0	44.0