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rps8-rpl14

LSC · 84724-84918 · 195 bp

Back to Commelina

Pi 0.0246

MarkerSeek score 61.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 7
pi: 0.0246
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGCTATTCGAACCGTTCCTT	AGTTTCATTGGCTCCCGAAG	45.0	50.0	57.2	58.2	289-304 bp	1.0	78.5
rps8-rpl14_p2	TGCTATTCGAACCGTTCCTT	TTTCATTGGCTCCCGAAGTC	45.0	50.0	57.2	58.2	287-302 bp	1.0	78.4
rps8-rpl14_p3	ATGCTATTCGAACCGTTCCT	AGTTTCATTGGCTCCCGAAG	45.0	50.0	57.0	58.2	290-305 bp	1.0	76.6
rps8-rpl14_p4	ATGCTATTCGAACCGTTCCT	TTTCATTGGCTCCCGAAGTC	45.0	50.0	57.0	58.2	288-303 bp	1.0	76.6
rps8-rpl14_p5	CGTAGATGCTATTCGAACCGT	AGTTTCATTGGCTCCCGAAG	47.6	50.0	58.0	58.2	295-310 bp	1.0	76.4