Hotspot

rpl20-rps12

LSC · 72326-73081 · 756 bp

Back to Commelina

Pi 0.00705
MarkerSeek score 63.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
756
variable_sites
8
pi
0.00705
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 GAGTGAGTCTCGAATGAGCC TCTCCCGCTCTTAGAGGATG 55.0 55.0 57.8 58.0 907-922 bp 1.0 79.1
rpl20-rps12_p2 ATCCCCGTCTTACTCTGGTC TCTCCCGCTCTTAGAGGATG 55.0 55.0 58.2 58.0 832-847 bp 1.0 79.0
rpl20-rps12_p3 GAGTGAGTCTCGAATGAGCC GTCACAAAATCTCCCGCTCT 55.0 50.0 57.8 57.9 916-931 bp 1.0 78.4
rpl20-rps12_p4 ATCCCCGTCTTACTCTGGTC GTCACAAAATCTCCCGCTCT 55.0 50.0 58.2 57.9 841-856 bp 1.0 78.3
rpl20-rps12_p5 GAGTGAGTCTCGAATGAGCC TGTCACAAAATCTCCCGCTC 55.0 50.0 57.8 58.2 917-932 bp 1.0 77.6