Hotspot

accD-psaI

LSC · 62848-63408 · 561 bp

Back to Commelina

Pi 0.0354

MarkerSeek score 71.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 26
pi: 0.0354
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	CCTGCGATCCCGTAAAAAGA	50.0	50.0	58.0	58.0	1261-1293 bp	1.0	85.5
accD-psaI_p2	GAAGGGGGTTTGGGTTTGAT	CCTGCGATCCCGTAAAAAGA	50.0	50.0	58.0	58.0	1588-1620 bp	1.0	84.3
accD-psaI_p3	GCCCCTCGTTATTGTATGCA	CCTGCGATCCCGTAAAAAGA	50.0	50.0	58.0	58.0	1631-1663 bp	1.0	84.1
accD-psaI_p4	AGACAGGGTTAACTGAGGCT	CCTGCGATCCCGTAAAAAGA	50.0	50.0	58.0	58.0	1791-1823 bp	1.0	83.8
accD-psaI_p5	GATCGGGGAACTTGGAATCC	CCTGCGATCCCGTAAAAAGA	55.0	50.0	58.0	58.0	1891-1923 bp	1.0	83.6